UNA is organized into individual locals based on the identity of the employer and each with its own executive and bylaws. UNA’s locals represent members in their own workplaces. As of 2012, there were more than 165 locals throughout Alberta, one in virtually every hospital and health centre. Locals range in size from well over 2,000 members to fewer than a dozen.

UNA locals are grouped into five districts. UNA district meetings are attended by the Presidents of all UNA locals in that specific district. District meetings are a unique opportunity to share information, problems and strategies. UNA locals are represented on the Executive Board by District Representatives.